Products Description
What is Hoechst 33342?
Bisbenzimide, also known as Hoechst 33342, is a commonly used reagent in cytogenetics research. It fluorescently stains the DNA of cells, inserts into the A-T region of DNA, has low cytotoxicity, and forms membrane-permeable fluorescent DNA chains. Hoechst 33342 is mainly used for DNA staining and blue fluorescent staining. It has low toxicity and is therefore widely used.


Basic Information
| CAS Number | 23491-52-3 |
| EINECS Number | 245-691-1 |
| Chemical Formula |
C27H28N6O |
| Molecular Weight | 452.55 |
| Density | 1.274 ± 0.06 g/cm³ (Predicted) |
| Boiling Point | 725.9 ± 70.0 °C (Predicted) |
| Solubility in Water | 20 mg/mL |
| Acid Dissociation Constant (pKa) | 10.97 ± 0.10 (Predicted) |
| Storage Condition | 2-8°C |
| Appearance | Yellow solid |
Advantages
- High membrane permeability, suitable for live cell staining. No need to fix cells and cells can remain active after staining, suitable for dynamic observation of cell behavior or real-time changes after drug treatment.
- Specific binding to DNA, pure fluorescence signal. Targeting double-stranded DNA, the cell nucleus shows bright blue fluorescence after staining, and the cytoplasm has almost no fluorescence, which makes it easy to clearly distinguish the cell nucleus and cytoplasm structures.
- Low cytotoxicity, suitable for long-term observation. Hoechst 33342 has extremely low toxicity and is suitable for combining with time-lapse photography technology to track cell cycle or stem cell differentiation process.
- Stable fluorescence signal and strong compatibility. Good resistance to photobleaching, compatible with multi-color fluorescence co-staining, and simultaneous detection of multiple parameters (such as cell nucleus + organelles + apoptosis markers) is achieved.
- Hoechst 33342 staining steps are simple and applicable to a variety of sample types.
- Compatible with flow cytometry.
Safety Information
- Do not breathe Hoechst 33342 dust.
- Wear appropriate protective clothing and gloves.
- Avoid contact with skin and eyes.
- In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
- In case of accident or if you feel unwell, consult a doctor immediately (show the label if possible).

Company Profile
Gneebio is a professional chemical raw material manufacturer and global distributor. Our company is located in Henan Province, China. We have multiple partner chemical factories in Henan, covering an area of 3,600 square meters, providing customers with a one-stop chemical procurement service, including mixing, repackaging, storage, and distribution.
Our hot-selling products include aromatic hydrocarbons (Naphtha (petroleum), Benzene, Toluene, o-Xylene, Styrene, Cyclohexane, Cyclohexanone, Epsilon-Caprolactam, 2-Anilinoethanol, etc.), alcohols (Ethylene Glycol, 1-Butanol, 2-Methyl-1-Propanol, Ethyl Alcohol, etc.), phenols and ketones (Phenol, Propafenone, Bisphenol A, Phenyl Methacrylate, Methyl Isobutyl Ketone, Phenolic Resin, etc.), and petrochemicals. We hope to establish long-term and mutually beneficial cooperation with more friends in the future and provide the best products and services for our customers.
Please contact us if you need any.




FAQ
Q:Can I have a sample for testing?
A: Yes! Testing allows you to visualize the effectiveness of the application. In fact, the samples are free of charge and you only need to pay for the courier fee.
Q:How about packaging?
A: It can be made according to your needs and the shape of your products. Generally speaking, we can provide iron drum packing, carton packing, wooden box packing and bag packing.
Q:What is your delivery time?
A: If the goods are in stock, it usually takes 5-7 working days. Or 15-20 working days after the order is placed.
Q:How to guarantee the after-sale service?
A:If there is any problem after sale, whether it is quality or quantity, we will try our best to solve it immediately, and we even have a recall system if necessary.
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